From francepampi at yahoo.it Thu Oct 14 05:34:51 2004 From: francepampi at yahoo.it (Francesca Pampinella) Date: Thu Oct 14 11:37:03 2004 Subject: [FRET] mounting medium for FRET Message-ID: <20041014093451.31064.qmail@web53110.mail.yahoo.com> I would like to have some information about ideal mounting media for confocal FRET imaging (acceptor photobleaching method). Thank you. --------------------------------- Nuovo Yahoo! Messenger E' molto pi? divertente: Audibles, Avatar, Webcam, Giochi, Rubrica Scaricalo ora! -------------- next part -------------- An HTML attachment was scrubbed... URL: http://six.pairlist.net/pipermail/fret/attachments/20041014/2d4d0033/attachment.html From crojas at spymac.com Mon Oct 18 15:52:55 2004 From: crojas at spymac.com (Cristian) Date: Mon Oct 18 15:57:12 2004 Subject: [FRET] mounting medium for FRET Message-ID: <20041018195258.5FC9D3805D@spy23.spymac.net> An HTML attachment was scrubbed... URL: http://six.pairlist.net/pipermail/fret/attachments/20041018/afdc5b4c/attachment.html From cammer at aecom.yu.edu Tue Oct 19 13:01:35 2004 From: cammer at aecom.yu.edu (Michael Cammer) Date: Tue Oct 19 13:05:35 2004 Subject: [FRET] mounting medium for FRET In-Reply-To: <20041018195258.5FC9D3805D@spy23.spymac.net> Message-ID: <5.2.1.1.2.20041019121248.02a6f640@mailserver.aecom.yu.edu> dsRed had a huge component of fluorescence in the green channel. Basically, you can't do it, I doubt even spectrally. At 01:52 PM 10/18/04 -0600, you wrote: >Hi, I would like to know if there is someone making FRET with dsRed and >GFP. I wish to obtain some good protocol because my proteins have a very >low expression level (cellular cycle) and dsRed (I read elsewhere) has a >weak brightness. Thanks >Any information will be appreciated > > > > > > >Cristian Rojas >Laborat?rio de Biologia Molecular de Plantas - DIPEQ >Instituto de Pesquisas Jardim Bot?nico do Rio de Janeiro >Rua Pacheco Le?o 915 >Jardim Bot?nico >Rio de Janeiro - RJ >Brasil > > > > >On Thu Oct 14 3:34 , Francesca Pampinella sent: > >I would like to have some information about ideal mounting media for >confocal FRET imaging (acceptor photobleaching method). > >Thank you. > > >Nuovo >Yahoo! Messenger E' molto pi? divertente: Audibles, Avatar, Webcam, >Giochi, Rubrica Scaricalo ora! > > > >---------- >Get Urchin 6 On Demand web analytics. Because you can't wait to be found. >http://www.urchin.com/?utm_campaign=U6OD&utm_medium=email&source=spymac.com&content=cantwait >_______________________________________________ >FRET mailing list >FRET@fretimaging.org >http://six.pairlist.net/mailman/listinfo/fret ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461 (718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/ **This electronic transmission contains information that is privileged.**